The Bielschowsky"s silver staining is commonly used to demonstrate axons, neurofibrillary tangles, nerve fibers and senile plaques. This staining technique improved on the method developed by Fajersztajn, who initially applied the silver mirror reaction to tissue staining. Max Bielschowsky a German neuropathologist, improved on Fajersztajn"s method by using a silver nitrate solution to incubate formalin-fixed frozen sections, followed by an ammoniacal silver solution, a reducing solution and a sodium thiosulfate solution to remove the unreduced silver. Early modifications of Bielschowsky method were summarized by Beech and Davenport. Further modifications were made by Garven and Gairns, Sevier and Munger, and Yamamoto-Hirano. However, these Bielschowsky staining procedures use relatively large volume of staining solutions, are costly, time-consuming, and difficult to achieve consistent results.

Hito Bielschowsky OptimStain™ Kit offers a simple solution to these problems. This kit is easy to use with simplified procedures, delivers stable and improved staining quality, with minimal overstains/background and artifacts when used properly. The users can process slides in large or small quantities without wasting the solutions. The developing step can be controlled slide by slide to achieve optimal staining and differentiation.

Hito Bielschowsky OptimStain™ Kit has been tested on the brains, spinal cords and peripheral nerves from several species of animals and proven to be sensitive for demonstrating the morphological details of axons and intracellular neurofibrils. It is a simple solution for your research.

Kit Contents	Standard Kit	Small Kit
	for 100 slides	for 40 slides
Solution-1	50 ml	20 ml
Solution-2	50 ml	20 ml
Solution-3	4 ml	2 ml
Solution-4	250 ml	125 ml
Hito Aqua Barrier PAP Pen (HTHS0110)	1	1
Staining Jar	2	1
User Manual and MSDS	1	1
Before using Hito Bielschowsky OptimStain™ Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat or MicrotomeDry ice, O.C.T. compound, isopentane, ethanol, xylene, double distilled or deionized waterPlastic/glass tubes or vialsSlide (recommend Hito Super-Safe slide Cat# HTHS0101) and coverslipsStaining jars for slides washResinous mounting mediumLight microscope Hito Bielschowsky OptimStain™ Kit Manual and MSDS